Forum

I'm working on a research project that involves the analysis of MSI/MSS status in H&E stained diagnostic slides from the COAD and READ cohort in the TCGA database. I've collected around 600 slides so far, but I've encountered some difficulties in finding information about the staining procedure used.

While most of the slides appear to have been stained using the standard H&E stain, some of the slides seem to be stained differently, with a more purple/blue hue.

Is this information about the staining procedure available in the TCGA database?
Are there any tools (like a python package or something) that could identify the stain type from the diagnostic slide?

Any suggestions or advice would be greatly appreciated.
Thanks